Pancreatic duct ligation in a minipig model leads to exocrine pancreatic insufficiency (EPI). This allows
the study of digestive processes and pancreatic enzyme replacement therapies. However, detailed descriptions of
the surgical procedure, perioperative management, a determination of exocrine pancreatic insufficiency are scarce in
the literature. Data of the long-term health status of minipigs upon EPI induction are still not available. Therefore, the
present study describes in detail an experimental approach to the induction of exocrine pancreatic insufficiency via
pancreatic duct ligation in minipigs and the long term follow up of the animal’s health state.